Signal Amplification
How our breakthrough technology works

Our unique value

SwiftDx specialise in unique lateral flow protein interactions that allow for the binding of large amount of specific proteins to low levels of target biomarkers

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Our products allow you to detect ultra-low levels of your biomarker of interest on a lateral flow test

eAMP (enhanced Amplification) can be easily customised for the chosen target illness

Our technology can also be easily incorporated into existing manufacturing processes

eAMP mode of action

eAMP molecules can be combined with a specific antibody to target the chosen antigen. Additional eAMP proteins specifically bind to the eAMP molecule preceding it, creating millions of available binding sites for visible molecules such as colloidal gold.

This specifically increases the sensitivity of antigen detection by up to 10 million times compared to direct colloidal gold binding alone.

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Demonstration of our technology

Figure shows nitrocellulose (NC) strips that have had different components passed up them. The NC has target antigen printed in 8 different lines which decrease in concentration on a log10 scale. The left axis gives the quantity of antigen on each line. Right axis gives an indication of the amount of amplification beyond direct targeting of a gold conjugate to the antigen. NS eAMP cascade control shows that proteins can flow through without causing background if the specific binding partner is not present.

Units: ng: nanogram; pg: picogram; fg: femtogram. Abbreviations: eAMP: enhanced amplification; NS: non-specific. 

This figure demonstrates how a single drop of eAMP amplifies the protein signal compared to direct colloidal gold staining. The more lines visible, the greater the amplification. 

With the double eAMP cascade, the signal is amplified up to 10 million times compared to direct colloidal gold staining. In comparison, the current standard (biotin amplification) only amplifies the signal up to 100 times more than direct staining alone.